Concentration Assay:Article Title: Development of 89 Zr-anti-CD103 PET imaging for non-invasive assessment of cancer reactive T cell infiltration
Article Snippet: .. Antibodies and reagents used in the study Antibody/reagents Dilutions/ concentration Catalog number/clone Vendor IL-2 6000 U/mL Proleukin Novartis recombinant TGFβ 10 ng/mL 100-21C Peprotech PHA 10 μg/mL L1668-5MG Sigma Aldrich Recombinant E-cadherin (1) 2 μg/mL 648-EC R&D systems Recombinant E-cadherin (1) 2 μg/mL 10204-H02H Sino Biological CD103 (2G5.1) antibody 10-20 μg/mL MCA708 Bio-rad CD103 (Ber-ACT8) antibody 10-20 μg/mL 550258 BD CD103 (Ber-ACT8)-FITC antibody 15 μL per test 561677 BD CD3-PE antibody 2 μL per test 12-0038-41/42 eBioscience CD8a-APC-eFlour 780 antibody 2 μL per test 47-0088-42 eBioscience CD33 -PE-Cy7 antibody 2 μL per test 25-0338-42 eBioscience CD3-PerCP-Cy5.5 antibody 2 μL per test 45-0037-42 Thermo Fisher Scientific doi: 10.1136/jitc-2022-004877:e004877. .. 10 2022;J Immunother Cancer, et al. Kol A CD324 (E-Cadherin) - PerCP-eFluor 710 antibody 1 μg/test 46-3249-82 eBioscience Mouse IgG1 κ Isotype Control 10-20 μg/mL 554721 BD biosciences Human IgG, Fab fragment 10-20 μg/mL 009-000-007 Jackson Immunoresearch
Article Title: Anti-CD103 antibodies
Article Snippet: .. The details of reagents and antibodies used for the following examples are provided in Table 1: Dilutions/ Catalog Antibody/reagents concentration number/clone Vendor IL-2 6000 U/mL Proleukin Novartis recombinant TGF 10 ng/mL 100-21C Peprotech PHA 10 μg/mL L1668-5MG Sigma Aldrich Recombinant E-cadherin (1) 2 μg/mL 648-EC R & D systems Recombinant E-cadherin (1) 2 μg/mL 10204-H02H Sino Biological CD103 (2G5.1) antibody 10-20 μg/mL MCA708 Bio-rad CD103 (Ber-ACT8) 10-20 μg/mL 550258 BD antibody CD103 (Ber-ACT8)-FITC 15 μL per test 561677 BD antibody CD3-PE antibody 2 μL per test 12-0038-41/42 eBioscience CD8a-APC-eFlour 780 2 μL per test 47-0088-42 eBioscience antibody CD33-PE-Cy7 antibody 2 μL per test 25-0338-42 eBioscience CD3-PerCP-Cy5.5 antibody 2 μL per test 45-0037-42 Thermo Fisher Scientific CD324 (E-Cadherin)- 1 μg/test 46-3249-82 eBioscience PerCP-eFluor 710 antibody Mouse IgG1 κ Isotype 10-20 μg/mL 554721 BD biosciences Control Human IgG, Fab fragment 10-20 μg/mL 009-000-007 Jackson Immunoresearch Zombie Aqua TM Fixable 1:100 423102 BioLegend Viability kit (ITK diagnostics) Goat anti-mouse Ig-PE 1:50 1010-9 Southern Biotech F(ab′)2 Fragment Goat 1:50 109-136-088 Jackson Anti-Human IgG (H + L)- Immunoresearch APC Murine CD3-FITC 2 μL per test 11-0032-82 eBioscience antibody Murine CD8a-PE-Cy7 2 μL per test 25-0081-82 eBioscience antibody Murine CD 103-PE 2 μL per test 12-1031-82 eBioscience antibody .. Chinese hamster ovary (CHO)-K1 cells and the human adenocarcinoma cell line MCF7 were obtained from the American Type Culture Collection (ATCC).
Recombinant:Article Title: Development of 89 Zr-anti-CD103 PET imaging for non-invasive assessment of cancer reactive T cell infiltration
Article Snippet: .. Antibodies and reagents used in the study Antibody/reagents Dilutions/ concentration Catalog number/clone Vendor IL-2 6000 U/mL Proleukin Novartis recombinant TGFβ 10 ng/mL 100-21C Peprotech PHA 10 μg/mL L1668-5MG Sigma Aldrich Recombinant E-cadherin (1) 2 μg/mL 648-EC R&D systems Recombinant E-cadherin (1) 2 μg/mL 10204-H02H Sino Biological CD103 (2G5.1) antibody 10-20 μg/mL MCA708 Bio-rad CD103 (Ber-ACT8) antibody 10-20 μg/mL 550258 BD CD103 (Ber-ACT8)-FITC antibody 15 μL per test 561677 BD CD3-PE antibody 2 μL per test 12-0038-41/42 eBioscience CD8a-APC-eFlour 780 antibody 2 μL per test 47-0088-42 eBioscience CD33 -PE-Cy7 antibody 2 μL per test 25-0338-42 eBioscience CD3-PerCP-Cy5.5 antibody 2 μL per test 45-0037-42 Thermo Fisher Scientific doi: 10.1136/jitc-2022-004877:e004877. .. 10 2022;J Immunother Cancer, et al. Kol A CD324 (E-Cadherin) - PerCP-eFluor 710 antibody 1 μg/test 46-3249-82 eBioscience Mouse IgG1 κ Isotype Control 10-20 μg/mL 554721 BD biosciences Human IgG, Fab fragment 10-20 μg/mL 009-000-007 Jackson Immunoresearch
Article Title: Anti-CD103 antibodies
Article Snippet: .. The details of reagents and antibodies used for the following examples are provided in Table 1: Dilutions/ Catalog Antibody/reagents concentration number/clone Vendor IL-2 6000 U/mL Proleukin Novartis recombinant TGF 10 ng/mL 100-21C Peprotech PHA 10 μg/mL L1668-5MG Sigma Aldrich Recombinant E-cadherin (1) 2 μg/mL 648-EC R & D systems Recombinant E-cadherin (1) 2 μg/mL 10204-H02H Sino Biological CD103 (2G5.1) antibody 10-20 μg/mL MCA708 Bio-rad CD103 (Ber-ACT8) 10-20 μg/mL 550258 BD antibody CD103 (Ber-ACT8)-FITC 15 μL per test 561677 BD antibody CD3-PE antibody 2 μL per test 12-0038-41/42 eBioscience CD8a-APC-eFlour 780 2 μL per test 47-0088-42 eBioscience antibody CD33-PE-Cy7 antibody 2 μL per test 25-0338-42 eBioscience CD3-PerCP-Cy5.5 antibody 2 μL per test 45-0037-42 Thermo Fisher Scientific CD324 (E-Cadherin)- 1 μg/test 46-3249-82 eBioscience PerCP-eFluor 710 antibody Mouse IgG1 κ Isotype 10-20 μg/mL 554721 BD biosciences Control Human IgG, Fab fragment 10-20 μg/mL 009-000-007 Jackson Immunoresearch Zombie Aqua TM Fixable 1:100 423102 BioLegend Viability kit (ITK diagnostics) Goat anti-mouse Ig-PE 1:50 1010-9 Southern Biotech F(ab′)2 Fragment Goat 1:50 109-136-088 Jackson Anti-Human IgG (H + L)- Immunoresearch APC Murine CD3-FITC 2 μL per test 11-0032-82 eBioscience antibody Murine CD8a-PE-Cy7 2 μL per test 25-0081-82 eBioscience antibody Murine CD 103-PE 2 μL per test 12-1031-82 eBioscience antibody .. Chinese hamster ovary (CHO)-K1 cells and the human adenocarcinoma cell line MCF7 were obtained from the American Type Culture Collection (ATCC).
Control:Article Title: Anti-CD103 antibodies
Article Snippet: .. The details of reagents and antibodies used for the following examples are provided in Table 1: Dilutions/ Catalog Antibody/reagents concentration number/clone Vendor IL-2 6000 U/mL Proleukin Novartis recombinant TGF 10 ng/mL 100-21C Peprotech PHA 10 μg/mL L1668-5MG Sigma Aldrich Recombinant E-cadherin (1) 2 μg/mL 648-EC R & D systems Recombinant E-cadherin (1) 2 μg/mL 10204-H02H Sino Biological CD103 (2G5.1) antibody 10-20 μg/mL MCA708 Bio-rad CD103 (Ber-ACT8) 10-20 μg/mL 550258 BD antibody CD103 (Ber-ACT8)-FITC 15 μL per test 561677 BD antibody CD3-PE antibody 2 μL per test 12-0038-41/42 eBioscience CD8a-APC-eFlour 780 2 μL per test 47-0088-42 eBioscience antibody CD33-PE-Cy7 antibody 2 μL per test 25-0338-42 eBioscience CD3-PerCP-Cy5.5 antibody 2 μL per test 45-0037-42 Thermo Fisher Scientific CD324 (E-Cadherin)- 1 μg/test 46-3249-82 eBioscience PerCP-eFluor 710 antibody Mouse IgG1 κ Isotype 10-20 μg/mL 554721 BD biosciences Control Human IgG, Fab fragment 10-20 μg/mL 009-000-007 Jackson Immunoresearch Zombie Aqua TM Fixable 1:100 423102 BioLegend Viability kit (ITK diagnostics) Goat anti-mouse Ig-PE 1:50 1010-9 Southern Biotech F(ab′)2 Fragment Goat 1:50 109-136-088 Jackson Anti-Human IgG (H + L)- Immunoresearch APC Murine CD3-FITC 2 μL per test 11-0032-82 eBioscience antibody Murine CD8a-PE-Cy7 2 μL per test 25-0081-82 eBioscience antibody Murine CD 103-PE 2 μL per test 12-1031-82 eBioscience antibody .. Chinese hamster ovary (CHO)-K1 cells and the human adenocarcinoma cell line MCF7 were obtained from the American Type Culture Collection (ATCC).
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